Fig. 4.

NR4A1 antagonists-induced changes in T-cell populations and transcription factors in CD8⁺ T-cells. Compound-induced changes on CD8⁺ and CD4⁺ T-cells (A) and Treg cells (B) in TILs was determined by flow cytometric analysis as outlined in Methods. C T-Bet, D NFAT1, and Tox/Tox2 expression in CD8⁺ T-cells was determined by flow cytometric analysis as outlined in Methods. Real-time PCR was used to determine mRNA levels of transcription factors (E), cytokines (F) and T-Bet (G) in CD8⁺ T-cells as outlined in Methods. Results are expressed as means ± SD. n = 5 for control and DIM-3,5-Cl₂ groups, n = 4 mice in DIM-3-Br-5-OCF₃ group. Significant (p values) induction or inhibition is indicated in graphs. The doses used were 2.5 mg/kg/d for both DIM-3-Br-5-OCF₃ and DIM-3,5-Cl₂