Fig. 5.

Flow cytometric analysis of CD8⁺ T-cells and surface markers associated with T-cell exhaustion in TILs from tumors. TILs were isolated from tumors derived from mice treated with corn oil (control), DIM-3-Br-5-OCF₃ (2.5 mg/kg/d) and DIM-3,5-Cl₂ (2.5 mg/kg/d). Flow cytometric analysis with specific antibodies were used to determine the percentage of CD4⁺ and CD8⁺ T TILs (A) and CD8⁺ TILs co-expressing PD1 and TIM3 and TILs expressing PD-1, 2B4, LAG3, TIM3, and TIGIT (B). The results are expressed as means ± SD. n = 5 for control and DIM-3,5-Cl₂ groups, n = 4 mice in DIM-3-Br-5-OCF₃ group. Significant (p values) induction or inhibition is indicated in graphs