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. 1998 Mar;180(5):1174–1184. doi: 10.1128/jb.180.5.1174-1184.1998

FIG. 4.

FIG. 4

Autoradiograph of RNA dot blots. RNA was purified from wild-type or nuo mutant cells, transferred directly to multiple nitrocellulose membrane, and probed with the nuo genes or fragments as indicated on the left. Lanes: 1, wild type (strain CP875); 2, wild type (CP366); 3, nuoB::Km mutant (AJW844); 4, nuoB-C::Cm mutant (AJW853); 5, nuoF::miniTn10Cm mutant (AJW851); 6, Δ(nuoF-L)-1 mutant (CP938); 7, nuoG::Tn10-1 mutant (CP910); 8, ΔnuoG1 mutant (AJW931); 9, nuoH::Km mutant (AJW845); 10, nuoI::Km mutant (AJW846); 11, nuoN::Km mutant (AJW847). The hybridization pattern for the nuoM::miniTn10Cm strain (AJW852) (data not shown) was identical to that for the nuoN::Km strain (AJW847) (lane 11). The hybridization pattern for AJW1516 was identical to that for AJW931 (data not shown).