TABLE 1.
Bacterial strains used in this study
| Strain | Relevant genotype | Source or reference |
|---|---|---|
| CP366 | polA(Ts) thi-1 thr(Am)-1 leuB6 his-4 rpsL136a lacY1 xyl-5 ara-14 tonA31 tsx-78 rha zig::Tn10a Ace− | 23 |
| CP875 | thi-1 thr(Am)-1 leuB6 his-4 rpsL136 lacY1 ΔlacX74 λlacY | 40 |
| AJW844 | CP875 nuoB::Km | MCN021 × CP875→Kmr Nuo− |
| AJW853 | CP875 nuoB-C::Cmb | MWC230 × CP875→Cmr Nuo− |
| AJW851 | CP875 nuoF::miniTn10Cmc | ZK1363 × CP875→Cmr Nuo− |
| CP938 | CP875 Δ(nuoF-L)-1 | 40 |
| CP910 | CP875 nuoG::Tn10-1a | 40 |
| CP932 | CP366 nuoG::Tn10-1 | 40 |
| AJW931 | CP366 ΔnuoG1d | This study |
| AJW1516 | CP875 ΔnuoG1 | This study |
| AJW1470 | CP366 nuoG2e | This study |
| AJW1517 | CP875 nuoG2 | This study |
| AJW845 | CP875 nuoH::Km | ND1-KanR × CP875→Kmr Nuo− |
| AJW846 | CP875 nuoI::Km | MCN091 × CP875→Kmr Nuo− |
| AJW852 | CP875 nuoM::miniTn10Cmc | ZK1362 × CP875→Cmr Nuo− |
| AJW847 | CP875 nuoN::Km | ANN141 × CP875→Kmr Nuo− |
| AJW932 | CP366 nuoΩ(pHF17)f | This study |
| AJW1459 | AJW931 nuoΩ(pAJW105ΔPstI)g | This study |
| AJW1472 | AJW1470 nuoΩ(pAJW105ΔPstI) | This study |
| AJW1582 | CP875 ΔnuoG1 nuoH::Km | This study |
| AJW1583 | CP875 ΔnuoG1 nuoI::Km | This study |
| AJW1584 | CP875 nuoG2 nuoH::Km | This study |
| MCN021 | nuoB::Km | 7 |
| MWC230 | nuoB-C::Cm | R. Gennis |
| ZK1363 | nuoF::miniTn10Cm | 59 |
| ND1-KanR | nuoH::Km | 7 |
| MCN091 | nuoI::Km | 7 |
| ZK1362 | nuoM::miniTn10Cm | 59 |
| ANN141 | nuoN::Km | 7 |
a
nuoG::Tn10-1 and zig::Tn10 confer Tcr; rpsL136 confers Strr.
b
The Cm cassette is located in the intergenic region between nuoB and nuoC.
c
This mutation has been renamed to reflect its location within the nuo locus.
d
The ΔnuoG1 mutation is a 235-bp deletion of a 3′ region of nuoG.
e
The nuoG2 mutation is a 235-bp tandem duplication of a 3′ region of nuoG.
f
This integration results in a duplication of the region nuoF-L and introduces the ΔnuoG1 allele (see Fig. 3). pHF17 confers Apr.
g
This integration results in a duplication of the region nuoF-L and introduces the wild-type nuoG allele (see Fig. 3). pAJW105ΔPstI confers Apr.