TABLE 2.
Summary of Nuo phenotypes
| Strain | nuo mutation(s) | Swarm plate resulta | TB growth curveb | Growth on M63 + 25 mM acetatec | NADH/d-NADH FeCN activitiesd | EPR spectroscopy resulte |
|---|---|---|---|---|---|---|
| CP875 | None (wild type) | + | + | + | + | + |
| CP366 | None (wild type) | + | + | NDf,g | + | + |
| AJW844 | nuoB::Km | − | − | − | −h | −h |
| AJW853 | nuoB-C::Cm | − | − | − | ND | ND |
| AJW851 | nuoF::miniTn10Cm | − | − | − | −h | ND |
| CP938 | Δ(nuoF-L)-1 | − | − | − | − | − |
| CP910 | nuoG::Tn10-1 | − | − | − | − | − |
| CP932 | nuoG::Tn10-1 | − | − | NDg | ND | ND |
| AJW931 | ΔnuoG1 | − | − | NDg | − | − |
| AJW1516 | ΔnuoG1 | − | − | − | ND | ND |
| AJW1470 | nuoG2 | − | − | NDg | ND | ND |
| AJW1517 | nuoG2 | − | − | − | ND | ND |
| AJW845 | nuoH::Km | − | − | − | −h | −h |
| AJW846 | nuoI::Km | − | − | − | −h | −h |
| AJW852 | nuoM::miniTn10Cm | − | − | − | ND | ND |
| AJW847 | nuoN::Km | − | − | − | −h | +h |
| AJW932 | CP366 nuoΩ(pHF17) | + | + | NDg | ND | ND |
| AJW1459 | AJW931 nuoΩ(pAJW105ΔPstI) | + | + | NDg | ND | ND |
| AJW1472 | AJW1470 nuoΩ(pAJW105ΔPstI) | + | + | NDg | ND | ND |
| AJW1582 | ΔnuoG1 nuoH::Km | − | − | − | ND | ND |
| AJW1583 | ΔnuoG1 nuoI::Km | − | − | − | ND | ND |
| AJW1584 | nuoG2 nuoH::Km | − | − | − | ND | ND |
a
+, presence of an inner, aspartate ring; −, absence of the inner ring.
b
+, no growth defect; −, growth defect.
c
+, good growth (≥1.0-mm colonies); −, poor growth (≤0.5-mm colonies).
d
+, wild-type levels of both activities in membrane fractions; −, less than 20% of wild-type activities.
e
+, at least three FeS centers detected; −, no detectable FeS centers.
f
ND, not done for this study.
g
The strain background was Ace− prior to introduction of the nuo mutation(s).
h
The mutation was analyzed in a different, but related, background.