Fig. 4. Cell apoptosis assessment of gill on exposure to different MP morphotypes over time.
A TUNEL assay showing cells with DNA damage (red) of each group, counterstained with DAPI (blue) for cell nucleus. Scale bar, 100 µm. B Box plots displaying quantification for TUNEL+ cells in the gill of each group. One-way ANOVA with Turkey’s post hoc test was performed to calculate significance (*p < 0.05, **p < 0.01, ***p < 0.001, n = 5). n/s; not significant.
