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. 2023 Dec 6;9:442. doi: 10.1038/s41420-023-01740-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Immunostaining for antibodies against DAPI (blue), pAKT (green), pERK (red) in the gill and DG of day 14–100 mg/L microsphere and microfiber groups. B Quantification for expression level of pERK and pAKT in gill and DG of each group. One-way ANOVA multiple comparison was performed to calculate significance (**p < 0.01, ***p < 0.001, n = 5). C Transcription level of target genes of the gill in day 14–100 mg/L microsphere and microfiber groups. Data are presented as mean ± SD. One-way ANOVA with Turkey’s post hoc test was conducted to calculate significance (*p < 0.05, **p < 0.01, ***p < 0.001, n = 3). n.s; not significant. D Schematic depicting the molecular pathways functioning in spherical and fibrous morphotypes MP-induced stress in mussels.