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. 2023 Dec 6;13:21549. doi: 10.1038/s41598-023-48795-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison between different methods to prepare Ni-MNPs for protein binding and purification. Three synthesis procedures in the literature were compared based on the steps that are required to synthesize (a) Ni-NTA MNPs⁴⁰ (b) glutardialdehyde MNPs (immobilization for non-his-tagged enzymes)⁴⁹ and (c) NiFe₂O₄ MNPs used in the current study. No washing or separation step was included.