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Alignment of deduced amino acid sequences from ISPα2NT (19), ISPαDNT (28), and ISPαNAP (19, 24). Differences in the ISPα amino acid sequences which may be responsible for the observed differences in substrate specificities between 2NTDO and 2,4-DNTDO are denoted by circled numbers. Conserved histidine and cysteine residues coordinating the [2Fe-2S] Rieske cluster are denoted by asterisks. Conserved amino acids thought to coordinate mononuclear iron are denoted by inverted triangles.
