TABLE 2.

Oxidation products formed by recombinant E. coli expressing 2NTDO, 2,4-DNTDO, NDO, and hybrid dioxygenases^a

Substrate    Product(s)	nmol of product/mg of total protein
	2NTDO	2,4-DNTDO	NDO	HD832	HD833	HD834
	10.64	ND	ND	ND	ND	0.04
	1.06	0.05	0.13	<0.01	0.04	<0.01
	0.13	ND	ND	ND	ND	0.03
	0.06	ND	ND	ND	ND	0.03
	0.03	0.23	0.25	<0.01	0.09	0.03
	0.16	0.08	ND	ND	0.05	0.01
	0.02	0.25	0.43	0.02	0.07	0.03
	ND	9.54	ND	0.37	0.75	ND
	0.16	ND	ND	ND	ND	0.05
	0.75	0.83	2.14	0.07	0.69	0.07

^aProducts were identified by comparisons to known standards as described in Materials and Methods. Amounts of oxidation products formed are given in nanomoles of product/milligram of total protein and are meant for comparison purposes, since biotransformations were not optimized for each individual substrate. ND, no product detected. <0.01, product detected but not quantified.