FIG. 4.

Southern analysis of RT-PCR products of the moa gene cluster of Synechococcus sp. strain PCC 7942, using a moaE gene probe. The primers used for the RT-PCR corresponded to DNA sequences of the moaA and moaC genes (see Materials and Methods for primers used). Lane 1, PCR-amplified strain PCC 7942 genomic DNA; lane 2, PCR-amplified, RNase-treated strain PCC 7942 total RNA; lane 3, RT-PCR-amplified RNA. Samples of lanes 2 and 3 were incubated with DNase before the RNase treatment or the retrotranscription reaction. The size of the amplified DNA fragment is indicated to the left.