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. 2023 May 1;14(3):341–353. doi: 10.32598/bcn.2021.2554.2

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Copyright© 2023 Iranian Neuroscience Society

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Figure 3. — Total protein separated by SDS-PAGE, Resolved on 12% resolving gel, stained with Commassie blue

Notes: A total of 10 wells of gel was loaded with 9 samples and protein marker (M) of known molecular weight to check the presence of protein and their resolution. The representative images of the protein blot of different areas of the brain and β-actin, for GLP-1 expression. At the bottom, a graphical representation of the GLP-1 protein expression is seen. Densitometric analysis was done using the Image J software by selecting bandwidth and measurement of intensity. The quantification of the bands was then performed using β-actin as a control. Each of the brain areas studied is shown on the X axis and the ratio of band intensities of the sample (from each brain area)/β-actin is shown on the Y axis.