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. 1998 Mar;180(5):1207–1214. doi: 10.1128/jb.180.5.1207-1214.1998

FIG. 2.

FIG. 2

SDS-PAGE analysis of the Yops (identified at left) secreted by wild-type Y. enterocolitica MRS40(pYV40) (lane 1), by MRS40(pMRS4071), an lcrVsycD mutant (lane 2), by MRS40(pMRS4071)(pMRS28), an lcrVsycD mutant complemented with lcrRGV (lane 3; note the overproduction of LcrV), by MRS40(pMRS4071)(pMRS72), an lcrVsycD mutant complemented with lcrGVsycD (lane 4), by MRS40(pMRS4071)(pPW64), an lcrVsycD mutant complemented with sycD (lane 5), by KNG22703(pPW2269), a nonpolar sycD mutant (lane 6; note that YopD and YopB do not appear in the absence of SycD [53]), and by KNG22703(pPW2269)(pPW64), an sycD mutant complemented with sycD (lane 7) (Note that YopB and YopD are present. LcrV [V] cannot be distinguished clearly. However, overexpression of SycD does not affect LcrV production and secretion [not shown].) Note that the size of YopM varies between strain E40 and strain W22703.