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. 1998 Mar;180(5):1207–1214. doi: 10.1128/jb.180.5.1207-1214.1998

FIG. 5.

FIG. 5

Binding of YopD, YopB, and LcrG to a GST-LcrV hybrid. (A) Western blot analysis with anti-LcrV and anti-YopD monoclonal antibodies of total-cell extracts and of the soluble extracts purified on glutathione-Sepharose beads from E. coli XL1(pCN29) overproducing GST and YopD (lanes 1) and XL1(pMRS84) overproducing GST-LcrV and YopD (lanes 2). (B) Western blot analysis with polyclonal anti-LcrV and monoclonal anti-YopB antibodies of the total-cell extracts and of the soluble extracts purified on glutathione-Sepharose beads from XL1(pCN40) overproducing GST, YopB, and SycD (lanes 1) and XL1(pCNG42) overproducing GST-LcrV, YopB, and SycD (lanes 2). (C) Western blot analysis with anti-LcrV and anti-LcrG polyclonal antibodies of the total-cell extracts and of the soluble extracts purified on glutathione-Sepharose beads from XL1(pMRS75) producing GST-LcrV and LcrG (lanes 1), XL1(pMRS78) producing GST-LcrVΔ224-266 and LcrG (lanes 2), and XL1(pMRS83) producing GST-LcrVΔ2-32 and LcrG (lanes 3).