Fig. 3.

Hepatic differentiation of hCdHO_C (hCdHO_C_DM). (A) Schematic of hepatic differentiation with hALO_C and hCdHO_C. (B) Relative mRNA levels of hepatic markers (ALB, HNF4a, AAT, ASGR1, CK19, CYP1A2, and CYP3A4) in hALO_C, hCdHO_C, hALO_C_DM, and hCdHO_C_DM determined by RT-qPCR. Expression levels of each gene marker in all samples were normalized agasint GAPDH (triplicate experiments). (C) Immunocytochemistry of hepatic markers (CK18 and ASGR1) in hALO_C_DM and hCdHO_C_DM. Scale bar: 50 μm. hCdHs, human chemically derived hepatic progenitors; hCdHO_C, hCdH-derived organoids in a collagen scaffold; DM, differentiation medium; hALO_C, hPH-derived organoids with collagen scaffold; EM, extension medium; ns, not significant. ***p < 0.001.