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ECL-Western analysis of τ and γ expression. W3110parE10 cells carrying various plasmids were grown in culture at 32°C, and ECL-Western analysis was performed as described in Materials and Methods. Lane 1, 15 ng of γ; lane 2, 15 ng of τ; lanes 3 to 8, W3110parE10 cells containing either pBR322 (lane 3), pBR-parE (lane 4), pBR-dnaX (lane 5), pBR-dnaXG230Op (lane 6), pBR-dnaX-γ (lane 7), or pBR-dnaX-τ (lane 8).
