FIG. 6.

Southern analysis of restricted chromosomal DNA of the S. aurantiaca wild-type strain DW4/3-1 and of the strains BS34 and BS35 with the fbfB gene and the neo gene as probes (Fig. 5). Lane 1, chromosomal DNA of BS34 restricted by EcoRI and SmaI. Three fragments (3.0, 2.8, and 1.7 kbp) were detected. Lane 2, chromosomal DNA of BS35 restricted by EcoRI and SalI. Two fragments (6.5 and 5.9 kbp) were detected. Lane 3, chromosomal DNA of the wild-type strain DW4/3-1 restricted by EcoRI and SmaI. Only a 3.6-kbp fragment was detected. Five micrograms of DNA per lane was analyzed. The sizes of the fragments were estimated by using HindIII-restricted λ DNA as a reference.