Northern analysis of RNA isolated from strain BS34 at different times after beginning of starvation with the 567-bp radiolabelled R. capsulatus pufBA fragment as a probe. A signal with the size of the pufBA fragment was detected after 14 h of starvation. Lane 1, RNA isolated from vegetative cells of BS34. Lanes 2 to 8, RNA isolated from cells 8, 10, 14, 16, 17, 18, and 24 h, respectively, after the beginning of starvation. A total of 10 μg of RNA from each sample was used. The sizes of the fragments were estimated with the RNA molecular weight marker I (Boehringer Mannheim).