TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| E. coli S17-1 | E. coli K-12 thi pro hsdR mutant hsdM+ recA (RP4 Smr Tpr) | 39 |
| S. aurantiaca strains | ||
| AP182 | DW4/3-1, fbfB::Tn5lacZ (Kmr Smr) | 29 |
| AP191 | DW4/3-1, fbf::Tn5lacZ (Kmr Smr) | 29 |
| BS14 | DW4/3-1, fbfA::neo (Kmr Smr) | 37 |
| BS34 | DW4/3-1, fbfB::(Δ5′Δ3′ fbfB-pufBA-neo) (merodiploid for fbfB) (Kmr Smr) | This work |
| BS35 | DW4/3-1, fbfB::(Δ3′ fbfB-Δtrp-lacZ-neo) (merodiploid for fbfB) (Kmr Smr) | This work |
| DW4/3-1 | Wild type (Smr) | 30 |
| Plasmids | ||
| pBS7 | fbfA::(pufBA-neo) in pBS SK(−) (Ampr Kmr) | 37 |
| pBS21 | pSUP102 harboring an XbaI-SalI fragment containing Δ3′Δ5′ fbfB-pufBA-neo (Cmr Kmr) | This work |
| pBS22 | pSUP102 harboring a HindIII-SalI fragment containing Δ3′ fbfB-Δtrp-lacZ-neo (Cmr Kmr) | This work |
| pSK24 | 3.6-kbp EcoRI-SmaI fragment containing fbfA and fbfB in pBS SK(−) (Ampr) | 37 |
| pBS SK(−) | Ampr | Stratagene |
| pSUP102 | Cmr Tetr | 38 |
| pSZ182 | pSUP102 containing a 20-kbp SalI fragment from AP182 harboring Tn5lacZ (Cmr Kmr) | 37 |
| pUC4KIXX | Ampr Kmr | Pharmacia |
| Mini-Tn5lacZ1 | Ampr Kmr | 5 |