Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Nov 23;14:1304466. doi: 10.3389/fimmu.2023.1304466

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 Ding, Zhao, Cai, Zhou, Chen, Bai, Liu, Liu and Zhou

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Silencing IRX3 Inhibits Proliferation, Invasion, and Metastasis while Promoting Apoptosis in Melanoma Cells. (A) A colony formation assay was performed on A375 and WM-115 melanoma cells in the NC and si-IRX3 groups. Smaller colonies were observed in the si-IRX3 group, indicating that IRX3 silencing inhibits melanoma cell proliferation. (B) Quantification of colony formation assay results showed a decrease in colony size in the si-IRX3 group compared to the NC group. (C) CCK-8 assay further confirmed the inhibitory effect of IRX3 silencing on melanoma cell proliferation. (D) Quantification of scratch assay results showing a decrease in wound closure percentage in the si-IRX3 group compared to the NC group. (E) Scratch assay revealed a decrease in 17 the migration ability of A375 and WM-115 cell in the si-IRX3 group compared to the NC group. (F) Transwell assay demonstrated a decrease in the invasion ability of A375 and WM-115 cells in the si-IRX3 group compared to the NC group. (G) Quantification of transwell assay results shows a decrease in the number of invading cells in the si-IRX3 group compared to the NC group. (H) Apoptosis assay revealed an increase in apoptosis in both A375 and WM-115 cell lines upon IRX3 silencing. (I) Quantification of apoptosis assay results shows an increase in the percentage of apoptotic cells in the si-IRX3 group compared to the NC group. *P < 0.05, **P < 0.01, ***P < 0.001.