TABLE 2.
Phenotypes of phase I variant, phase II variant, and cip mutants of P. luminescens NC1
| Phenotype assayed | Phenotypea of strain:
|
|||
|---|---|---|---|---|
| NC1/1 (phase I variant) | NC1/2 (phase II variant) | NP151 (cipB1::Ω-Km) | NP173 (cipA1::Ω) | |
| Dye adsorption | ||||
| Neutral red | + | w+ | w+ | w+ |
| Eosin Y-methylene blue | + | − | w+ | w+ |
| Bromthymol blue | + | − | − | − |
| Congo red | + | − | − | − |
| Bioluminescence | + | − | − | − |
| Extracellular products | ||||
| Lipase activity | + | − | − | − |
| Hemolytic activity | + | − | w+ | w+ |
| Siderophore activity | + | − | w+ | w+ |
| Protease activity | + | − | − | − |
| Antibiotic production | + | − | + | w+ |
| Biochemical traits | ||||
| Indole production | + | − | − | − |
| Citrate utilization | + | − | + | + |
| Glucose utilization | − | + | + | + |
| Colony morphology on: | ||||
| 2% PP3 agar | Convex, mucoid | Flat, nonmucoid | Convex, mucoid | Convex, mucoid |
| Antibiotic medium no. 3 | Flat, rough | Flat, rough | Flat, rough | Flat, spreading |
| Pigmentation on: | ||||
| 2% PP3 agar | Yellow | Light yellow | Yellow | Yellow |
| 0.5% Tween 20 agar | Yellow | Nonpigmented | Nonpigmented | Nonpigmented |
| 0.5% Tween 60 agar | Yellow | Light yellow | Light yellow | Light yellow |
| Egg yolk agar | Yellow | Yellow | Orange | Orange |
| CAS agar | Yellow | White | White | White |
| Support of nematode growth and reproduction | + | − | − | − |
a
Assayed as described in Materials and Methods. +, positive; −, negative; w+, weakly positive.