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. 2023 Oct 30;15(12):e17737. doi: 10.15252/emmm.202317737

Figure EV5. ZBTB16 action in ER‐positive breast cancer.

Figure EV5

  1. Boxplot depicting ZBTB16 mRNA expression in normal breast tissues, Luminal A, as well as Luminal B cancer samples (TCGA cohort n = 702). The box begins in the first quartile (25%) and ends in the third (75%), while the line represents the median value. The lines represent segments to furthest data without accounting for outliers.
  2. Overall survival probabilities of breast cancer patients (KMplotter meta dataset; n = 1,310) grouped by ZBTB16 mRNA expression (Luminal A high expression = purple, Luminal A low expression = gray, and Luminal B = blue). Overall survival probabilities in months are plotted for each group, and censored patients are shown as vertical tick marks.
  3. Progression‐free and overall survival probabilities of breast cancer patients (METABRIC dataset) grouped by ZBTB16 mRNA expression (Luminal A high expression = purple, Luminal A low expression = gray, and Luminal B = blue). Progression‐free and overall survival probabilities in months are plotted for each group, censored patients are shown as vertical tick marks.
  4. Western blot showing expression of V‐5‐tagged GFP and ZBTB16. n = 3.
  5. Normalized cancer cell viability for MCF‐7 GFP (green) and ZBTB16 (orange) overexpression models. n = 4.
  6. Representative crystal violet assay image for MCF‐7 GFP and ZBTB16 overexpression models. n = 3.
  7. Upper panel, volcano plot depicting differences in protein expression between GFP‐OE and ZBTB16‐OE models in MCF‐7 cells. Significance is depicted as a color gradient; lower panel, intensity (LFQ) values per category (decrease in expression, same levels, and increase in expression upon ZBTB16 overexpression). n = 4. The box begins in the first quartile (25%) and ends in the third (75%), while the line represents the median value. The lines represent segments to furthest data without accounting for outliers.
  8. Volcano plot depicting differences in protein expression between GFP‐OE and ZBTB16‐OE models in EFM‐192A cells. n = 4.
  9. Reactome cell cycle (gene‐set reference: R‐HSA‐1640170) GSEA enrichment profiles based on whole‐proteome data comparison between GFP‐OE and ZBTB16‐PE in MCF‐7 cell line.
  10. Hallmark E2F targets (gene‐set reference: M5925) GSEA enrichment profiles based on whole‐proteome data comparison between GFP‐OE and ZBTB16‐PE in EFM‐192A cell line.
  11. Volcano plot depicting differentially enriched (over IgG control) interactors in ZBTB16‐RIME experiments in MCF‐7 cells. n = 4. P‐values were determined by two‐sided t‐test.
  12. GSEA enrichment profiles for “Reactome Signaling by nuclear receptors” gene sets based on IgG versus ZBTB‐16 RIME comparison (n = 4).

Data information: All experiments were performed in biological replicates and the number (n) of replicates indicated.