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. 2023 Nov 24;95(48):17733–17740. doi: 10.1021/acs.analchem.3c03595

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© 2023 The Authors. Published by American Chemical Society

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O-PTIR reveals variations in the intracellular PHB content between B. sphaericus B0769 cells. (a) O-PTIR spectra acquired from 16 individual B. sphaericus B0769 cells from within the same population. (b) PCA score plot obtained from these O-PTIR spectra; values in parentheses represent the percentage of the total explained variance (TEV). (c) PC-1 loading plots obtained from O-PTIR data acquired from 16 B. sphaericus B0769 cells (green) compared to PC-1 loadings calculated from input data containing O-PTIR spectra from all 14 Bacillus strains (red). (d) Ester carbonyl/amide I area ratio calculated from infrared signatures from B. sphaericus B0769 cells. (e) Correlation between ester carbonyl/amide I area ratio and PC-1. Plots in (a) are offset for clarity and arranged in increasing PHB content from the 1727 cm^–1/1656 cm^–1 area ratio.