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. 2023 Nov 23;14:1282710. doi: 10.3389/fimmu.2023.1282710

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Copyright © 2023 Lui, Ye, Chan, Tanaka and Cheung

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Vδ2 T cells induce pyroptosis in mesothelioma cells. (A) Analysis of protein expression level of gasdermin (Gas)E, caspase 3, GasD, caspase 4, and (B) IL-18, following 6 h of co-culture between Vδ2 T cells and MSTO-luc. Controls include raptinal and terfenadine, or cells only. Arrows indicate expected band size of the full-length or cleaved proteins. Numbers under the bands represent band intensity normalized to β-actin. Representative immunoblots are shown. (C) ELISA analysis of IL-1β release following 6 h of co-culture between Vδ2 T cells and MSTO-luc at 10:1 ratio. plotted as a column graph of mean ± SEM. Data from three independent experiments are shown. One-way ANOVA statistical test was used. *P< 0.05.