Fig. 4.

Activation defects of Cbx4‐deficient T cells. Naive CD4⁺ and CD8⁺ T cells from WT (wild‐type) and KO (knockout) mice were stimulated with anti‐CD3 (2 μg·mL⁻¹) and anti‐CD28 (1 μg·mL⁻¹) for 72 h. (A–D) Cell proliferation was measured by BrdU incorporation (A). Cell survival was analyzed with Annexin V and 7‐AAD staining (n = 4) (B). IL‐2 and IFN‐γ production by CD4⁺ T cells (n = 4) (C) and IFN‐γ, GzmB, and TNF‐α expression by CD8⁺ T cells (n = 4) (D) was examined by flow cytometry. Representative histograms (left) and statistical data (right) from three to four independent experiments are shown. (E–G) Body weight of Rag1 ^−/−recipient mice given wild‐type or Cbx4 KO CD4⁺CD45RB^hi naïve T cells were adoptively transferred into Rag1 ^−/−recipient mice (n = 3 for each group). Body weight was monitored weekly (E). At the end of experiment, colon length was recorded (F) and the colonic sections were examined by HE staining (scale bar = 1 cm) (G). Scale bar = 100 μm. Data are presented as Mean ± SE. Unpaired t‐test was used for comparison, *P < 0.05, **P < 0.01, ***P < 0.001.