TABLE 8.
Pho regulon control in ΔphoU mutants by using ParaB-phoB+, PrhaB-phoB+, and PrhaB-phoR+ fusions
| Strain | Relevant genotypea | Growthb | BAP sp act onc:
|
|
|---|---|---|---|---|
| −Ind | +Ind | |||
| BW17142 | ΔphoU | +/− | 419d | N.D. |
| BW17335 | Δ(pstSCAB-phoU) | +++ | 428d | N.D. |
| BW18897 | ΔphoU | + | 444 ± 33 | N.D. |
| BW24739 | Δ(pstSCAB-phoU) Δ(ackA pta) | +++ | 443 ± 20 | N.D. |
| BW24774 | ParaB-phoB+ ΔphoB Δ(pstSCAB-phoU) Δ(ackA pta) | ++++ | 0.2 ± 0.0 | 94.8 ± 3.5 |
| BW24950 | ParaB-phoB+ ΔphoB ΔphoU Δ(ackA pta) | ++++ | 0.2 ± 0.0 | 113 ± 3 |
| BW24773 | PrhaB-phoB+ ΔphoB Δ(pstSCAB-phoU) Δ(ackA pta) | ++++ | 0.2 ± 0.0 | 109 ± 27 |
| BW24949 | PrhaB-phoB+ ΔphoB ΔphoU Δ(ackA pta) | ++++ | 0.2 ± 0.0 | 89.7 ± 6.4 |
| BW24768 | PrhaB-phoR+ ΔphoR Δ(pstSCAB-phoU) Δ(ackA pta) | ++++ | 0.2 ± 0.0 | 104 ± 2 |
| BW24948 | PrhaB-phoR+ ΔphoR ΔphoU Δ(ackA pta) | ++++ | 0.2 ± 0.0 | 83.4 ± 8.9 |
a
Complete genotypes are given in Table 1. The ParaB-phoB+ and PrhaB-phoB+ fusions are in single copy at attλ; the PrhaB-phoR+ fusion is in single copy at attHK022.
b
Colony size after 24 h of incubation +/−, barely detectable; +, tiny; +++, ca. 1 mm in diameter; ++++, ca. 1.5 mm in diameter.
c
Cells were assayed after 18 h of growth in 0.06% mannitol–MOPS–2 mM Pi without (−Ind) or with (+Ind) arabinose or rhamnose for induction. BAP specific activity values are as defined for Table 5. N.D., not determined.
d
BAP specific activity value is an average of four independent determinations (unpublished data cited in reference 26).