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Schematic drawing of constructs used to transform S. cerevisiae YSH 2.64-1A. (A) Construct 112CK14 contains the cDNA insert of A. foetidus 1-SST without modifications. (B) Construct 112CK14L contains a 70-bp 5′ untranslated leader sequence of the spinach sucrose transporter cloned into the PstI site. (C) Construct 112CK14S carries the vacuole-targeting sequence of the potato patatin gene B33 as a translational fusion to the CK14 coding region. Prom., promoter; Term., terminator.
