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. 1998 Mar;180(5):1305–1310. doi: 10.1128/jb.180.5.1305-1310.1998

FIG. 3.

FIG. 3

(Left panel) Seminative SDS-PAGE analysis of protein preparations from A. foetidus. Lanes: 1, ammonium sulfate precipitate of culture filtrate; 2, ConA eluate with 0.25 M α-d-methylmannoside; 3, Mono Q chromatography with 150 to 205 mM KCl; 4, Superdex 200 fraction at 48 to 49 min; M, molecular mass markers with sizes in kilodaltons indicated on the right. (Middle panel) Denaturing (lane 1) versus seminative (lane 2) SDS-PAGE analyses of A. foetidus 1-SST. The Superdex 200 fraction (5 μg) at 48 to 49 min was loaded per lane. M, molecular mass markers, with sizes in kilodaltons indicated on the right. (Right panel) Deglycosylation of A. foetidus 1-SST with N-glycosidase F. Lanes: 1, deglycosylated purified A. foetidus 1-SST; 2, untreated control; 3, N-glycosidase F; M, molecular mass markers, with sizes in kilodaltons indicated on the right.