TABLE 1.
Purification step | Total activitya | Protein concn (mg) | Sp actb | Purification factor |
---|---|---|---|---|
Culture medium | 85.2 | 29.38 | 2.9 | |
(NH4)2SO4 pellet | 64 | 20.03 | 3.2 | 1.1 |
ConA | 31.3 | 2.27 | 13.8 | 4.69 |
Mono Q | 27.9 | 0.34 | 82.1 | 27.93 |
Superdex 200 | 23.6 | 0.092 | 257.2 | 87.5 |
Total activity is expressed in micromoles of glucose min−1 at 37°C.
Specific activity is expressed in micromoles of glucose μg of protein−1 min−1 at 37°C.