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. 2023 Oct;73(5):335–345. doi: 10.30802/AALAS-CM-23-000035

Table 2.

Pn prevalence of zebrafish after the pilot study

Number of fish exposed Number of fish that died during the study Number of fish that tested positive for Pn after 28-d pilot study Prevalence P a
ALB 10 1 1 11% > 0.99
FUM 10 0 0 0% > 0.99
Control 10 0 1 10%
Total 30 1 2 7%

Prevalence was calculated as the number of Pn-infected fish identified on whole-body qPCR divided by the total number of live fish at the end of the 28-d pilot study. One fish that died from the ALB group in the pilot study was excluded from the calculations of ALB group prevalence and total prevalence.

a

Fisher exact tests were used to analyze results from either the ALB or FUM groups compared with those of the infected control group. A P < 0.05 was considered significant.