Table 3. Average Diameter, Polydispersity Index, and Zeta Potential Results of the Optimized Cationic Liposomes Containing Buffer or Cas9 after Storage of 90 Days at 4 °C^a.

formulation	storage duration (days)	average diameter (nm ± SD.)	PI (±SD.)	ZP (mV ± SD.)
Lipo/⌀	0	166.4 ± 0.52	0.102 ± 0.016	32.5 ± 1.56
	7	169.2 ± 1.93	0.112 ± 0.009	32.2 ± 0.92
	14	166.7 ± 1.71	0.104 ± 0.005	32.3 ± 1.56
	30	168.5 ± 1.47	0.131 ± 0.015	31.0 ± 1.35
	60	181.9 ± 1.54	0.203 ± 0.208	29.5 ± 1.97
	90	184.1 ± 4.05	0.242 ± 0.032	33.1 ± 1.25
Lipo/Cas9	0	190.7 ± 0.56	0.160 ± 0.007	36.6 ± 1.25
	7	187.4 ± 0.81	0.133 ± 0.024	32.9 ± 0.49
	14	189.4 ± 2.52	0.155 ± 0.017	36.0 ± 1.29
	30	202.7 ± 1.65	0.212 ± 0.009	36.2 ± 1.15
	60	435.9 ± 16.7	0.490 ± 0.040	15.7 ± 0.51
	90	1557.1 ± 208.5	0.272 ± 0.087	3.48 ± 1.70
Lipo/Cas9:sgRNA (N+/P– molar ratio)	0	229.1 ± 3.66	0.089 ± 0.013	25.7 ± 0.87

^aAD = average diameter; PI = polydispersity index; ZP = zeta potential; SD = standard deviation.