TABLE 3.

Nitrate uptake and nitrate reductase activity in K. oxytoca^a

Strain	Genotype	Nitrate uptakeb (nmol of NO2− min−1 mg [DW]−1)	Nitrate reductasec (nmol of NO2− min−1 mg [DW]−1)
VJSK2214	nasFED+	9.2	10.2
VJSK2217	ΔnasF132	4.6	12.4
VJSK2218	ΔnasE133	<0.1	11.3
VJSK2219	ΔnasD134	<0.1	10.5

^aAll strains are ΔnasB Δ(narKG). 

^bGlucose-dependent nitrate uptake rates in intact cells were determined over a 10-min time course as described in Materials and Methods. 

^cReduced methyl viologen-dependent nitrate reductase enzyme activities in toluene-permeabilized cells were determined over a 10-min time course as described in Materials and Methods.