TABLE 2.
MAbs which react with Opa proteins
| MAba | Immunoglobulin subclass | Minimal epitope | Specificity |
|---|---|---|---|
| O623 | G2a | NKS | HV1-1 |
| U506 | G1 | NKS | HV1-1 |
| O521b | G3 | NRQD | HV1-1 |
| T116 | NDc | TWKEL | HV1-7 |
| L614 | G1 | KESNYS | HV1-9 |
| P219b | G1 | IY | HV2-2 |
| P322b,d | G3 | TIYNQ | HV2-2 |
| P414b | G2a | TPTIYNQ | HV2-2 |
| W320/15 | G2a | NIP | HV2-2 |
| W124 | ND | NIP | HV2-2 |
| W104 | ND | PTNIPGGT | HV2-2 |
| AB419 | G2b | TTFL | HV2-6 |
| W320/16 | ND | PSGSTT | HV2-8 |
| U205 | G2b | PVPQGPTPK | HV2-10 |
| O516/2b | G2b | KGATQ | HV2-14 |
| P110b,d | G2b | KPTKGAT | HV2-14, HV2-19 |
| P112b,d | G2a | QP | HV2-14 |
| N312 | G1 | QP | HV2-14 |
| P515b,d | G3 | KGAT | HV2-14 |
| P416b,d | G2a | PTKGATQP | HV2-14 |
| P514b,d | G2a | PTKGATQP | HV2-14 |
| 210/G9 | G2a | QPGKL | HV2-14, HV2-19 |
| 192/B8 | G1 | TQPGKLV | HV2-14, HV2-46 |
a
All have been previously described by Achtman et al. (1) except for D309 (not shown) (2), O521 (18), and 192/B8, 210/G9, L614, O623, and U506 (described for the first time in this report).
b
Strong immunofluorescence with bacteria expressing Opa proteins recognized by the MAb.
c
ND, not determined.
d
Bactericidal at concentration of <8 μg/ml.