Fig. 2 |. BIRs mediate histone and DNA binding functions of BIRC2.

a,b, Western blot analysis of pulldowns of the GST-tagged BIR domains of BIRC2 with the indicated histone peptides. RBP2-PHD, control. The experiments were performed independently three times. c,d, Overlays of ¹H,¹⁵N HSQC spectra of the BIR domains of BIRC2 collected before (black) and after addition of the indicated peptides. Spectra are color-coded according to the protein:peptide molar ratio. e–g, Overlays of ¹H,¹⁵N HSQC spectra of the mutated BIRC2 BIR domains collected before (black) and after addition of the indicated peptides. Spectra are color-coded according to the protein:peptide molar ratio. h,i, Western blot analysis of pulldowns of the GST-tagged mutated BIRC2 BIR domains with the indicated histone peptides. The experiments were performed independently twice. j, Alignment of a fragment of the amino acid sequences of the BIRC2 BIR domains. Identical residues are highlighted by red boxes and moderately conserved residues are colored red. BIRC2_BIR3 residues are labeled. k–m, EMSAs of 147 bp 601 DNA in the presence of increasing amounts of the indicated BIR domains of BIRC2. DNA:protein ratio is shown below gel images.