. 2023 Nov 15;62(23):3383–3395. doi: 10.1021/acs.biochem.3c00381

Table 1. Kinetic Parameters of SIRT2^cat and SIRT2^cat(Q142A/E340A) for Acylated Peptides^a.

	SIRT2^cat			SIRT2^cat(Q142A/E340A)
H4K16 peptide modification	k_cat (min^–1)	K_m (μM)	k_cat/K_m (μM^–1 min^–1)	k_cat (min^–1)	K_m (μM)	k_cat/K_m (μM^–1 min^–1)
acetyl	18.5 ± 1.0	5.7 ± 0.9	3.25	4.9 ± 0.2	9.4 ± 1.1	0.52
4-oxononanoyl	0.4 ± 0.1	2.9 ± 1.0	0.14	0.2 ± 0.1	6.2 ± 2.8	0.03
decanoyl	1.5 ± 0.1	0.4 ± 0.1	3.75	1.5 ± 0.1	0.8 ± 0.1	1.88
dodecanoyl	1.1 ± 0.1	0.9 ± 0.3	1.22	1.1 ± 0.1	1.5 ± 0.4	0.73
myristoyl	0.7 ± 0.1	0.8 ± 0.3	0.88	0.6 ± 0.1	0.8 ± 0.4	0.75
	k_cat, NAD+ (min^–1)	K_m, NAD+ (μM)	k_cat/K_m, NAD+ (μM^–1 min^–1)	k_cat, NAD+ (min^–1)	K_m, NAD+ (μM)	k_cat/Km, NAD+ (μM^–1 min^–1)
acetyl	28.5 ± 2.4	234.1 ± 52.4	0.12	11.9 ± 0.8	317.5 ± 53.5	0.04

Parameters for each of the five modifications were determined using 1 mM NAD⁺ (Figure 2A–E). Also determined were parameters for NAD⁺ using 16 μM acetyl peptide (Figure 2F). Standard error is shown.

Table 1. Kinetic Parameters of SIRT2cat and SIRT2cat(Q142A/E340A) for Acylated Peptidesa.

Table 1. Kinetic Parameters of SIRT2^cat and SIRT2^cat(Q142A/E340A) for Acylated Peptides^a.