Table 11.

Influence of nicotine on ROS generation.

Effect = ↓ ↑	Assay	Exposure time	Tested concentration [mmol/l]	Effective concentration [mmol/l]	IC50/EC50 [mmol/l]	Level of significance (P-value)	Number of exp.	Authors
=	luminol turnover, chemilumimetric	3h	0.01 - 1	−	−	−	n.d.	Hosseinzadeh et al., 2016 (50)
=	luminol turnover, chemilumimetric	30min	0.0062 – 0.062▵	−	−	−	6	Matthews et al., 2011 (54)
=	isoluminol turnover, chemilumimetric	30min	0.0062 – 0.062▵	−	−	−	6	Matthews et al., 2011 (54)
↓	DCFH turnover, spectrophotometric	20min	0.0001 - 0.0003▵	0.0002 - 0.0003	−	0.0002 mmol/l: < 0.05 0.0003 mmol/l: < 0.01	6	Aspera-Werz et al., 2022 (45)
=	luminol turnover, chemilumimetric	60min	0.49▵	−	−	NS	3	Al-Shibani et al., 2011 (55)
P. gingivalis-stimulated =	luminol turnover, chemilumimetric	60min	0.49▵	−	−	−	3	Al-Shibani et al., 2011 (55)
F. nucleatum-stimulated =	luminol turnover, chemilumimetric	150min	0.0062 – 0.062▵	−	−	−	6	Matthews et al., 2011 (54)
F. nucleatum-stimulated =	isoluminol turnover, chemilumimetric	150min	0.0062 – 0.062▵	−	−	−	6	Matthews et al., 2011 (54)
OZA-stimulated ↓	luminol turnover, chemilumimetric	10min	0.01 - 0.5	0.05 - 0.5	−	0.05 mmol/l: < 0.05 0.1 - 0.5 mmol/l: < 0.01	4	Sasagawa et al., 1984 (74)
fMLP/CB-stimulated ↓	chemilumimetric	5min	1 - 10	10	IC50 = 3	< 0.001	5	Srivastava et al., 1989 (70)
O2 - =	lucigemin turnover, chemilumimetric	30min	0.0062 - 0.062▵	−	−	−	5	Matthews et al., 2012 (52)
O2 - ↓	cytochrome C turnover, spectrophotometric	10min	0.000031 - 15	3.1 - 15	−	< 0.01	3	Totti et al., 1984 (73)
O2 - ↑	cytochrome C turnover, spectrophotometric	5-90min	3	3	−	< 0.01	3	Iho et al., 2003 (56)
O2 -, ops S. aureus-stimulated =	lucigemin turnover, chemilumimetric	150min	0.0062 - 0.062▵	−	−	−	4	Matthews et al., 2012 (52)
O2 -, F. nucleatum-stimulated =	lucigemin turnover, chemilumimetric	150min	0.0062 - 0.062▵	−	−	−	4	Matthews et al., 2012 (52)
O2 -, LPS-stimulated =	lucigemin turnover, chemilumimetric	150min	0.0062 - 0.062▵	−	−	−	4	Matthews et al., 2012 (52)
O2 -, PMA-stimulated =	ferricytochrome C turnover, spectrophotometric	n.s.	0.0005 - 1.5	−	−	−	3	Seow et al., 1994 (64)
O2 -, PMA-stimulated ↑	cytochrome C turnover, spectrophotometric	10min	0.00001 - 0.01	0.00001 - 0.01	−	< 0.05	5	Jay et al., 1986 (71)
O2 -, fMLP-stimulated ↑	cytochrome C turnover, spectrophotometric	10min	0.00001 - 0.01	0.00001 - 0.01	−	< 0.05	5	Jay et al., 1986 (71)
O2 -, fMLP-stimulated ↓	cytochrome C turnover, spectrophotometric	10min	0.000031 - 15	3.1 - 15	−	< 0.01	3	Totti et al., 1984 (73)
O2 -, fMLP-stimulated/CB ↓	ferricytochrome C turnover, spectrophotometric	2min	0.005 - 0.5	0.05 - 0.5	−	0.05 mmol/l: < 0.05 0.1 - 0.5 mmol/l: < 0.01	5	Sasagawa et al., 1985 (72)
O2 -, fMLP-stimulated/CB (↓)	ferricytochrome C turnover, spectrophotometric	2min	0.1	(0.1)	−	n.d.	4	Sasagawa et al., 1985 (72)
O2 -, fMLP stimulated ↑	cytochrome C turnover, spectrophotometric	24.5h	0.001	0.001	−	< 0.05	3	Aoshiba et al., 1996 (62)
O2 -, LPS/PMA-stimulated ↓	cytochrome C turnover, spectrophotometric	7min	0.62 - 6.2 ✦	0.62 - 6.2	−	< 0.001	2	Pabst et al., 1995 (63)
O2 2-, PMA-stimulated ↓	phenol red turnover, spectrophotometric	1h	1.86 - 6.2 ✦	1.86 - 6.2	−	< 0.05	2	Pabst et al., 1995 (63)
H2O2 ↑	amplex red turnover, spectrophotometric	25min - 15h	2.5	2.5	−	increase of > 1.2-fold	2	Iho et al., 2003 (56).
↑	DHR123 stain, flow cytometry	25min - 12h	2.5	2.5	−	increase of > 1.2-fold	2	Iho et al., 2003 (56)
C5a-stimulated ↓	DHR123 stain, flow cytometry	5min	0.00001 - 1	0.001 - 1	−	0.001 mmol/l: < 0.001 0.01 - 1 mmol/l: < 0.05	5	Vukelic et al., 2013 (19)
Fcy-receptor cross-linked ↓	DHR123 stain, flow cytometry	5min	0.001	0.001	−	< 0.05	5	Vukelic et al., 2013 (19)

= no effect; ↓ effect on neutrophils inhibited by nicotine; ↑ effect on neutrophils strengthened by nicotine. ✦ Nicotine concentrations declared by the author. ▵ Nicotine concentration converted in to molar values based on the molar mass of nicotine of 162.23 g/mol. n.d., not determined; NS, not significant.