Table 3.
Influence of nicotine on apoptosis.
| Effect = ↓ ↑ |
Assay | Exposure time | Tested concentration [mmol/l] |
Effective concentration [mmol/l] |
IC50/EC50 [mmol/l] |
Level of significance (P-value) |
Number of exp. |
Authors |
|---|---|---|---|---|---|---|---|---|
| = | DNA gel electrophoresis | 8h | 0.5 - 22 | − | − | − | n.s. | Yoshida et al., 1998 (59) |
| ↓ | acridine orange, ethidium bromide stain, fluorescence microscopy | 24/48/72h | 0.001 | 24h: 0.001 | − | 24h: < 0.05 ✧ | 6 | Aoshiba et al., 1996 (62) |
| ↓ | electron microscopy | 24/48/72h | 0.001 | 24h: 0.001 | − | 24h: < 0.05 ✧ | 6 | Aoshiba et al., 1996 (62) |
| (↓) | DNA gel electrophoresis | 24h | 0.001 | (0.001) | − | − | 1 repr. | Aoshiba et al., 1996 (62) |
| ↑ | DAPI stain, fluorescence microscopy | 30min | 0.6 – 18.5▵ | 18.5 | − | 7h: < 0.05 ✧ | 3 | Mariggio et al., 2001 (58) |
| (↑) | DNA gel electrophoresis | 30min | 0.6 – 18.5▵ | − | − | − | 1 repr. | Mariggio et al., 2001 (58) |
| Fas ↓* | mRNA, RT-qPCR | 12h | 0.001 | 0.001 | − | 0.001 | 3 | Tyagi et al., 2021 (46) |
| Fas (↑) | ELISA | 30min | 0.6 – 18.5▵ | (18.5) | − | n.d. | 3 | Mariggio et al., 2001 (58) |
= no effect; ↓ effect on neutrophils inhibited by nicotine; ↑ effect on neutrophils strengthened by nicotine. Specified subpopulation of *non adherent neutrophils in culture. ✧ In case of kinetic studies, only the time points with effects are shown. ▵ Nicotine concentration converted into molar values based on the molar mass of nicotine of 162.23 g/mol. n.d., not determined; n.s. not stated; repr., representative result shown.