Skip to main content
. 2023 Nov 23;14:1281685. doi: 10.3389/fimmu.2023.1281685

Table 4.

Influence of nicotine on chemotaxis.

Effect
= ↓ ↑
Assay Exposure time Tested
concentration
[mmol/l]
Effective
concentration
[mmol/l]
IC50/EC50
[mmol/l]
Level of
significance
(P-value)
Number of
exp.
Authors
towards fMLP = traversing agarose 2h during movement 0.0001 - 0.1 n.d. 2 - 4 Gala et al., 1984 (75)
towards fMLP ↑ traversing agarose 90min preincubation 0.0001 - 0.01 0.0001 - 0.01 0.0001 mmol/l: < 0.05
0.001 - 0.1mmol/l:
< 0.01
5 Nowak et al., 1990 (68)
towards fMLP ↓ traversing agarose 90min preincubation 0.1 - 10 10 < 0.01 5 Nowak et al., 1990 (68)
towards fMLP ↑ microchemotaxis chambers 24h preincubation 0.001 0.001 < 0.01 5 Aoshiba et al., 1996 (62)
towards fMLP ↑ modified Boyden Chamber n.s. 0.00003 - 0.0308 0.00031 - 0.0308 < 0.001 15 Totti et al., 1984 (73)
towards fMLP ↑ modified Boyden Chamber n.s. 0.0308 0.0308 < 0.01 15 Totti et al., 1984 (73)
towards fMLP = modified Boyden Chamber 45min during movement 0.005 - 0.5 5 Sasagawa et al., 1985 (72)
towards fMLP ↓ traversing agarose 15min preincubation + 2h during movement 0.005 - 1.5 1 - 1.5 < 0.001 3 Seow et al., 1994 (64)
towards C5fr ↑ modified Boyden Chamber n.s. 0.00003 - 0.0308 0.00031 - 0.0308 < 0.001 15 Totti et al., 1984 (73)
towards ZAS ↑ traversing agarose 90min preincubation 0.0001 - 0.01 0.0001 - 0.01 0.0001 mmol/l: < 0.05
0.001 mmol/l: < 0.01
0.01 mmol/l: < 0.05
5 Nowak et al., 1990 (68)
towards ZAS ↓ traversing agarose 90min preincubation 0.1 - 10 1 - 10 < 0.01 5 Nowak et al., 1990 (68)
toward LTB4 ↓ traversing agarose 15min preincubation + 2h during movement 0.005 - 1.5 1 - 1.5 < 0.001 3 Seow et al., 1994 (64)
towards CXCL8 ↓ traversing agarose 15min preincubation + 2h during movement 0.005 - 1.5 1 - 1.5 < 0.001 3 Seow et al., 1994 (64)
toward CaAS ↓ traversing agarose 15min preincubation + 2h during movement 0.005 - 1.5 1 - 1.5 < 0.001 3 Seow et al., 1994 (64)
towards EAS ↓ modified Boyden Chamber 2h during movement 2 - 10 6 - 10 IC50 = 3.5 < 0.05 3 - 6 Bridges et al., 1977 (76)
towards nicotine (↑) traversing agarose 90min 0.0001 - 10 (0.0001-10) n.d. 9 Nowak et al., 1990 (68)

= no effect; ↓ effect on neutrophils inhibited by nicotine; ↑ effect on neutrophils strengthened by nicotine. n.d., not determined; n.s., not stated.