Table 5.
Influence of nicotine on general motility.
| Effect = ↓ ↑ |
Assay | Exposure time | Tested concentration [mmol/l] |
Effective concentration [mmol/l] |
IC50/EC50 [mmol/l] |
Level of significance (P-value) |
Number of exp. |
Authors |
|---|---|---|---|---|---|---|---|---|
| Undirected movement (↓) | traversing agarose | 90min preincubation | 0.0001 - 10 | (0.01 – 10) | − | n.d. | n.s. | Nowak et al., 1990 (68) |
| Undirected movement ↑ | trans-well system | 2h during movement | 0.6 | 0.6 | − | < 0.01 | 3 | Iho et al., 2003 (56) |
| Undirected movement (↑) | modified Boyden Chamber | n.s. | 0.000031 - 10 | (0.031 - 1) | − | n.d. | 15 | Totti et al., 1984 (73) |
| Undirected movement = | modified Boyden Chamber | 45min during movement | 0.005 - 0.5 | − | − | – | 5 | Sasagawa et al., 1985 (72) |
| Filtration time = | cell filterability assay | 10min | 0.001 | − | − | − | n.s. | Aoshiba et al., 1994 (66) |
|
Filtration time,
O2-stimulated = |
cell filterability assay | 10min | 0.001 | − | − | − | 5 | Aoshiba et al., 1994 (66) |
|
Filtration time,
fMLP stimulated = |
cell filterability assay | 2min | 0.001 | − | − | − | 5 | Aoshiba et al., 1994 (66). |
|
Filtration time,
chloramineT-induced ↓ |
cell filterability assay | 10min | 0.001 | 0.001 | − | < 0.05 | 5 | Aoshiba et al., 1994 (66). |
|
Filtration time,
H2O2-induced ↓ |
cell filterability assay | 10min | 0.001 | 0.001 | − | < 0.05 | 5 | Aoshiba et al., 1994 (66) |
| Actin filament ↑ | flow cytometry | 30sec, 30min | 0.1 - 10 | 1-10 | − | < 0.05, < 0.01 | 3 | Ryder et al., 1994 (65) |
|
Actin filament,
FLPEP-stimulated ↑ |
flow cytometry | 30sec | 10 | 10 | − | < 0.05 | 3 | Ryder et al., 1994 (65) |
= no effect; ↓ effect on neutrophils inhibited by nicotine; ↑ effect on neutrophils strengthened by nicotine. n.d., not determined; n.s., not stated.