Table 7.
Influence of nicotine on eicosanoid synthesis.
| Effect = ↓ ↑ |
Assay | Exposure time | Tested concentration [mmol/l] |
Effective concentration [mmol/l] |
IC50/EC50 [mmol/l] |
Level of significance (P-value) |
Number of exp. |
Authors |
|---|---|---|---|---|---|---|---|---|
| LTB4, A23187-stimulated ↓ | secreted, HPLC | 15min | 0.0000005 - 0.5 | 0.0000005 - 0.5 | IC50 = 0.0321 | < 0.05 | 6 | Saareks et al., 1993 (67) |
| LTB4, A23187-stimulated ↑ | secreted, enzyme immunoassay | 15min | 0.0005 - 1.5 | 1 - 1.5 | − | < 0.001 | 3 | Seow et al., 1994 (64) |
| LTB4, A23187-stimulated ↑ | secreted, isotope-labelled GC-MS | 1/3/8/12h | 0.000062▵ | 0.000062 | − | < 0.05 | 5 | Loke et al., 2012 (53) |
| LTB4, cotinine/A23187-stimulated (↑) | secreted, isotope-labelled GC-MS | 1/3/8/12h | 0.000062▵ | (0.000062) | − | n.d. | 5 | Loke et al., 2012 (53) |
| PGE2, A23187-stimulated ↑ | secreted, radio immunoassay | 15min | 0.0000005 - 0.5 | 0.0000005 - 0.5 | IC50 = 0.0365 | < 0.05 | 6 | Saareks et al., 1993 (67) |
| PGE2, concanavalin A-stimulated ↑ | secreted, enzyme immunoassay | 15min | 0.0005 - 1.5 | 0.5 - 1.5 | − | 0.5 mmol/l: < 0.01 1 - 1.5 mmol/l: < 0.001 |
3 | Seow et al., 1994 (64) |
= no effect; ↓ effect on neutrophils inhibited by nicotine; ↑ effect on neutrophils strengthened by nicotine. ▵ Nicotine concentration converted in to molar values based on the molar mass of nicotine of 162.23 g/mol. n.d., not determined.