TABLE 1.
Strains and plasmids used in this work
| Strain or plasmid | Genotype or features | Background | Source |
|---|---|---|---|
| Streptomyces coelicolor | |||
| HU261 | bld261 hisA1 uraA1 strA1 NF SCP2* | J. Willey | |
| LS17 | bldA39 hisA1 uraA1 strA1 Pgl− | K. Chater | |
| NS17 | bldK1::aadA | Lab collection | |
| NS40 | bldK1::aad hisA1 uraA1 strA1 | This study | |
| NS47 | bld261 hisA1 uraA1 strA1 NF SCP2* pJRM10 | This study | |
| NS48 | bld261 hisA1 uraA1 strA1 NF SCP2* pbldK22 | This study | |
| NS49 | bld261 bldK1::aadA hisA1 uraA1 strA1 NF SCP2* pJRM10 | This study | |
| NS50 | bld261 bldK1::aadA hisA1 uraA1 strA1 NF SCP2* pbldK22 | This study | |
| Escherichia coli | |||
| DH5α | supE44 ΔlacU169 (φ80lacZΔM15)hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Lab collection | |
| ER-2 | F′ lacIq leuB6 thi-1 fhuA31 lacY1 tsx-78 galK2 galT22 supE44 hisG4 rpsL136 (Strr) xyl-5 mtl-1 dam13::Tn9(Cmr) dcm-6 mcrB1 mcrA hsdR2(rK− mK+) | J. McCormick | |
| Plasmids | |||
| pJRM10 | bla tsr | pIISK+ and pIJ911 | Lab collection |
| pbldK22 | bldKABCDE tsr | pJRM-10 | Lab collection |