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ATP-PPi exchange activity of FenB
| Amino acid | Exchange activity (cpm)a | % Activity |
|---|---|---|
| Isoleucine | 107,038 | 100 |
| Glutamic acid | 284 | 0.26 |
| Alanine | 3,370 | 3.1 |
| Proline | 1,384 | 1.2 |
| Ornithine | 1,582 | 1.4 |
| Threonine | 2,171 | 2.0 |
| Tyrosine | 1,834 | 1.7 |
| Valine | 13,630 | 12.7 |
| Blank | 1,497 | 1.4 |
The reaction mixture consisted of 84 μg of recombinant FenB. The reaction was allowed to proceed for 10 min.
