Loss of neutral trehalase activity causes an osmotic-stress-resistant phenotype in S. pombe. (A) Growth of strains JY742 (control) (▪), MMT-3 (Δntp1::ura4+) (•), PBU13 (Δtps1::ura4+) (○), and MMT-4 (ura4+) (□) in YES medium supplemented with 0.3 M NaCl. (B) Strains JY742 (control), MMT-8 (Δntp1::ura4+), and MMT-4 (ura4+) were grown in YES medium to mid-log phase, and approximately 300 viable cells were seeded on YES or YES plus 0.2 M NaCl agar plates that were incubated at 28°C for 10 days.