TABLE 1.
Trehalose content in control and ntp1-disrupted strains during heat shock and under osmotic stress
| Straina (relevant genotype) | Trehalose contentb (nmol/107 cells) after:
|
||
|---|---|---|---|
| No treatment | Heat shock | Osmotic stress | |
| JY742 (control) | 3.5 ± 0.3 | 73.3 ± 4.0 | 21.2 ± 1.2 |
| MMT-3 (Δntp1::ura4+) | 7.2 ± 0.9 | 138.6 ± 9.2 | 57.7 ± 6.4 |
| MMT-8 (Δntp1::ura4+) | 7.7 ± 0.5 | 129.1 ± 6.7 | 53.1 ± 5.5 |
a
Mid-exponential-phase cultures growing on YES medium were extracted for trehalose content measurement before and after either a 60-min treatment at 40°C (heat shock) or the addition of 0.75 M NaCl (osmotic stress).
b
Values are means ± standard deviations of at least three independent determinations for each strain.