Figure EV2. Knockdown of tumour‐derived Gbb and ImpL2 rescues muscle OPP and ECM protein Nidogen.

• A
  Tumour qPCRs showing mRNA expression levels of gbb in Ras ^V12 dlg1 ^RNAi larvae expressing mCherry ^RNAi , ImpL2 ^RNAi , Gbb ^RNAi in the tumour (n = 3).
• B
  Quantification of normalised muscle OPP intensity in w ¹¹¹⁸ and Ras ^V12 dlg1 ^RNAi tumour‐bearing animals, where mCherry ^RNAi is specifically expressed in the tumour. w ¹¹¹⁸ (n = 15) and Ras ^V12 dlg1 ^RNAi (n = 15).
• C, D
  OPP staining detecting protein translation in the muscles of Ras ^V12 dlg1 ^RNAi tumour‐bearing animals that express lacZ ^RNAi ; mCherry ^RNAi or ImpL2 ^RNAi ; gbb ^RNAi .
• E
  Quantification of OPP in the muscles of wild type w ¹¹¹⁸ (n = 18) and tumour‐bearing animals expressing LacZ ^RNAi ;mCherry ^RNAi (n = 16), ImpL2 ^RNAi ; lacZ ^RNAi (n = 14), gbb ^RNAi ; mCherry ^RNAi (n = 8), ImpL2 ^RNAi ; gbb ^RNAi (n = 15).
• F
  Quantification of normalised muscle Nidogen intensity in w ¹¹¹⁸ and Ras ^V12 dlg1 ^RNAi tumour‐bearing animals, where mcherry ^RNAi is specifically expressed in the tumour. w ¹¹¹⁸ (n = 14) and Ras ^V12 dlg1 ^RNAi (n = 13)
• G, H
  Nidogen staining detecting ECM localisation in the muscles of Ras ^V12 dlg1 ^RNAi tumour‐bearing animals that express lacZ ^RNAi ; mCherry ^RNAi or ImpL2 ^RNAi ;gbb ^RNAi .
• I
  Quantification of Nidogen in wild type w ¹¹¹⁸ (n = 10) and tumour‐bearing animals expressing LacZ ^RNAi ;mCherry ^RNAi (n = 11), ImpL2 ^RNAi ; lacZ ^RNAi (n = 16), gbb ^RNAi ; mCherry ^RNAi (n = 10), ImpL2 ^RNAi ; gbb ^RNAi (n = 14).

Data information: Scale bar is 50 μm. Graphs are represented as Mean ± SEM, n = the number of samples. (*) P < 0.05 (**) P < 0.01, (***) P < 0.001, (****) P < 0.0001, (ns) P > 0.05. For experiments with two genotypes, two‐tailed unpaired student's t‐tests were used to test for significant differences. The Welch's correction was applied in cases of unequal variances. For experiments with more than two genotypes, significant differences between specific genotypes were tested using a one‐way ANOVA and a subsequent Šidák post‐hoc test.