Figure 3. Insulin signalling in the fat body improves muscle integrity in cachectic animals.

• A, B
  Schematic depicting intact muscle fillet in control animals, versus deteriorated muscle fillets in tumour animals.
• C, D
  Muscle fillets stained with phalloidin (Actin) from w ¹¹¹⁸ or QRas ^V12 scrib ^RNAi tumour‐bearing animals where mCherry ^RNAi was expressed in the fat body (r4‐GAL4). Detachments are marked with yellow arrows.
• E
  Quantification of normalised muscle detachment in (C, D). w ¹¹¹⁸ (n = 3), QRas ^V12 scrib ^RNAi (n = 10).
• F, G
  Muscle fillets stained with phalloidin (Actin) from QRas ^V12 scrib ^RNAi tumour‐bearing animals raised at 25°C, where in the fat body, either mCherry ^RNAi , or Akt was overexpressed.
• H
  Quantification of normalised muscle detachment in (F, G), r4>mCherry ^RNAi (n = 3). QRas ^V12 scrib ^RNAi ; r4>mCherry ^RNAi (n = 5), QRas ^V12 scrib ^RNAi ; r4>Akt (n = 8).
• I, J
  Muscle fillets stained with phalloidin (Actin) from QRas ^V12 scrib ^RNAi tumour‐bearing animals raised at 25°C, where in the fat body, either lacZ;mCherry ^RNAi , or InR ^CA ; mCherry ^RNAi was overexpressed.
• K
  Quantification of normalised muscle detachment in (I, J), r4>mCherry ^RNAi (n = 3). QRas ^V12 scrib ^RNAi ; r4>lacZ;mCherry ^RNAi (n = 3), QRas ^V12 scrib ^RNAi ; r4>InR ^CA ; mCherry ^RNAi (n = 9).
• L–N
  Muscle fillets of QRas ^V12 scrib ^RNAi tumour‐bearing animals raised at 25°C where InR ^CA ; mCherry ^RNAi or mad ^RNAi ; LacZ ^RNAi or InR ^CA ; mad ^RNAi was expressed in the fat body (r4‐GAL4).
• O
  Quantification of muscle detachment in (L–N). w ¹¹¹⁸ (n = 10), InR ^CA ; mCherry ^RNAi (n = 8) or mad ^RNAi ; LacZ ^RNAi (n = 14) or InR ^CA ; mad ^RNAi (n = 9).

Data information: Scale bar is 200 μm for muscle fillet staining carried out at 7 days after tumour induction. Graphs are represented as Mean ± SEM, n = the number of samples. (***) P < 0.001, (****) P < 0.0001, (ns) P > 0.05. For experiments with two genotypes, two‐tailed unpaired student's t‐tests were used to test for significant differences. The Welch's correction was applied in cases of unequal variances. For experiments with more than two genotypes, significant differences between specific genotypes were tested using a one‐way ANOVA and a subsequent Šidák post‐hoc test.

Source data are available online for this figure.