-
A, B
Muscle fillets stained with phalloidin (Actin) where luciferase or Akt was expressed under the control of MHC‐GAL4 in QRas
V12
scrib
RNAi
tumour‐bearing animals.
-
C
Quantification of normalised muscle detachment in (A, B). Wilt type control (MHC>mCherry
RNAi
, n = 3), UAS‐luciferase (n = 10), Akt (n = 17).
-
D, E
Muscle segment (outlined) where luciferase or Akt was expressed under the control of MHC‐GAL4 in QRas
V12
scrib
RNAi
tumour‐bearing animals.
-
F
Quantification of normalised muscle width/length in (D, E). Wild type control (n = 15), luciferase (n = 9), Akt (n = 16).
-
G, H
Muscle Nidogen staining where luciferase or Akt was expressed under the control of MHC‐GAL4 in QRas
V12
scrib
RNAi
tumour‐bearing animals.
-
I
Quantification of normalised muscle Nidogen staining in (G, H). luciferase (n = 16), Akt (n = 15).
-
J, K
Muscle segment from QRas
V12
scrib
RNAi
tumour‐bearing animals, where mCherry
RNAi
or Akt was expressed in the fat body (r4‐GAL4).
-
L
Quantification of normalised muscle width/length in (J, K). r4>mCherry
RNAi
(n = 9), QRas
V12
scrib
RNAi
;r4>mCherry
RNAi
(n = 5), QRas
V12
scrib
RNAi
;r4>Akt (n = 8).
-
M, N
Muscle segment from QRas
V12
scrib
RNAi
tumour‐bearing animals, where mCherry
RNAi
or mad
RNAi
was expressed in the fat body (r4‐GAL4).
-
O
Quantification of normalised muscle width/length in (M, N). r4>mCherry
RNAi
(n = 11), QRas
V12
scrib
RNAi
; r4>mCherry
RNAi
(n = 18), QRas
V12
scrib
RNAi
; r4>mad
RNAi
(n = 15).
-
P
Quantification of normalised muscle OPP intensity where luciferase (n = 10) or Akt (n = 16) was expressed under the control of MHC‐GAL4 in QRas
V12
scrib
RNAi
tumour‐bearing animals.
-
Q, R
Muscle from Ras
V12
dlg1
RNAi
tumour‐bearing animals expressing either mCherry
RNAi
or ImpL2
RNAi
in the tumour, where TGF‐ß signalling activation is indicated by pMad staining.
-
S
Quantification of normalised muscle pMad intensity in (Q, R). w
1118
(n = 30), mCherry
RNAi
(n = 30), ImpL2
RNAi
(n = 26).
-
T
Quantification of normalised muscle detachment where MHC>mCherry
RNAi
(n = 5) or mCherry
RNAi
(n = 9) or lacZ
RNAi
; mad
RNAi
(n = 6) was expressed under the control of MHC‐GAL4 in QRas
V12
scrib
RNAi
tumour‐bearing animals.