Figure 5. Fat body insulin signalling negatively regulates TGF‐ß signalling.

Schematic on the left depicts the insulin‐Pi3K signalling pathway, where the components manipulated are labelled with numbers and are referred to below.

• A, B
  Fat body from fed and starved animals where Dilps level is reduced (1) with TGF‐ß signalling activation indicated by pMad staining, dissected at day 5 after larval hatching (ALH).
• C
  Quantification of normalised pMad staining in (A, B). Fed (n = 7), starved (n = 20).
• D, E
  Fat body from animals raised at 18°C, dissected at day 7 ALH, that express mCherry ^RNAi or InR ^CA (2) under the control of CG‐GAL4, with TGF‐ß signalling activation indicated by pMad staining.
• F
  Quantification of normalised pMad staining in (D, E). mCherry ^RNAi (n = 30), InR ^CA (n = 40).
• G, H
  Fat body from animals expressing mCherry ^RNAi or p60 (3) under the control of CG‐GAL4, with TGF‐ß signalling activation indicated by pMad staining, dissected at day 5 ALH.
• I
  Quantification of normalised pMad staining in (G, H). mCherry ^RNAi (n = 30), p60 (n = 30).

Data information: Scale bar is 50 μm. Graphs are represented as Mean ± SEM, n = the number of samples. (**) P < 0.01, (***) P < 0.001, (****) P < 0.0001, two‐tailed unpaired student's t‐tests were used to test for significant differences. The Welch's correction was applied in cases of unequal variances.

Source data are available online for this figure.