Figure 6. Fat body insulin signalling negatively regulates ECM accumulation.

• A, B
  Viking::GFP localisation in fat body from animals expressing mCherry ^RNAi or TOR ^DN under the control of r4‐GAL4.
• C
  Quantification of normalised Viking::GFP intensity in (A, B). mCherry ^RNAi (n = 10) TOR ^DN (n = 9).
• D
  Quantification of normalised muscle detachment caused by the expression of mCherry ^RNAi or TOR ^DN under the control of r4‐GAL4. mCherry ^RNAi (n = 8) TOR ^DN (n = 7).
• E, F
  Viking::GFP localisation in fat body from animals expressing mCherry ^RNAi or p60 under the control of r4‐GAL4.
• G
  Quantification of normalised Viking::GFP intensity in (E, F). mCherry ^RNAi (n = 9), p60 (n = 10).
• H
  Quantification of normalised muscle detachment caused by the expression of mCherry ^RNAi or p60 under the control of r4‐GAL4. mCherry ^RNAi (n = 12), p60 (n = 16).
• I–K
  Viking::GFP localisation in fat body from animals expressing p60; mCherry ^RNAi or lacZ ^RNAi ; mad ^RNAi or p60; mad ^RNAi under the control of r4‐GAL4.

Data information: Scale bar is 50 μm for fat body staining, dissected at day 5 ALH. Graphs are represented as Mean ± SEM, n = the number of samples. (*) P < 0.05 (****) P < 0.0001, (ns) P > 0.05, two‐tailed unpaired student's t‐tests were used to test for significant differences. The Welch's correction was applied in cases of unequal variances.

Source data are available online for this figure.