Figure 7. Fat body TOR signalling negatively regulates TGF‐ß signalling.

Schematic on the left depicts Insulin/Tor signalling pathway, where the components manipulated are labelled with numbers and are referred to below.

• A, B
  Fat body from animals expressing mCherry ^RNAi or TOR ^DN (1) under the control of CG‐GAL4, with TGF‐ß signalling activation indicated by pMad staining.
• C
  Quantification of normalised pMad (to DAPI) staining in (A, B). mCherry ^RNAi (n = 21), TOR ^DN (n = 14).
• D, E
  Fat body from animals expressing mCherry ^RNAi or 4E‐BP ^CA (4) under the control of CG‐GAL4, with TGF‐ß signalling activation indicated by pMad staining.
• F
  Quantification of normalised pMad staining in (D, E). mCherry ^RNAi (n = 15), 4E‐BP ^CA (n = 15).
• G, H
  Fat body from animals expressing mCherry ^RNAi or S6K ^CA (2) under the control of CG‐GAL4, with TGF‐ß signalling activation indicated by pMad staining.
• I
  Quantification of normalised pMad staining in (G, H). mCherry ^RNAi (n = 20), S6K ^CA (n = 30).
• J, K
  Fat body from animals expressing mCherry ^RNAi or FOXO (3) under the control of CG‐GAL4, with TGF‐ß signalling activation indicated by pMad staining.
• L
  Quantification of normalised pMad staining in (J, K). mCherry ^RNAi (n = 30), FOXO (n = 30).
• M–P
  Fat body from animals expressing mCherry ^RNAi or Tor ^DN (1) or InR ^CA ; mCherry ^RNAi (5) or InR ^CA ;Tor ^DN (5, 1) under the control of r4‐GAL4, with TGF‐ß signalling activation indicated by pMad staining (the same mCherry ^RNAi sample was used in Fig 8A, as these experiments were carried out together).
• Q
  Quantification of normalised pMad staining in (M–P). mCherry ^RNAi (n = 16), Tor ^DN (n = 20), InR ^CA ; mCherry ^RNAi (n = 12), InR ^CA ;Tor ^DN (n = 16). The same mCherry ^RNAi and InR ^CA ; mCherry ^RNAi data points were used as in Fig 8F.

Data information: Scale bar is 50 μm, dissection carried out at 5 days ALH. Graphs are represented as Mean ± SEM, n = the number of samples. (*) P < 0.05 (**) P < 0.01, (****) P < 0.0001, (ns) P > 0.05. For experiments with two genotypes, two‐tailed unpaired student's t‐tests were used to test for significant differences. The Welch's correction was applied in cases of unequal variances. For experiments with more than two genotypes, significant differences between specific genotypes were tested using a one‐way ANOVA and a subsequent Šidák post‐hoc test.

Source data are available online for this figure.